Table 2 The information of primary antibodies in Western Blotting
Name of Antibodies | Brand and Item Number | Dilution | Molecular weight (kDa) |
|---|---|---|---|
LAGE3 | Biorbyt orb327020 | 1: 500 | 14 |
Ras | CST 3339 | 1: 1000 | 21 |
Raf | Abcam ab137435 | 1:3000 | 73 |
PI3K | Abcam ab86714 | 1:1000 | 85 |
P-Akt (Ser473) | CST 4060 | 1: 1000 | 60 |
Akt (Pan) | CST 4691 | 1: 1000 | 60 |
P-MEK1 | CST 9127 | 1:1000 | 45 |
MEK1 (p-S298) | CST 2352 | 1:1000 | 45 |
p-Erk | CST 4376 | 1: 1000 | 42, 44 |
Erk (MAPK1) | CST 4695 | 1: 1000 | 42, 44 |
Braf | Abcam ab33899 | 1: 2000 | 90 |
GAPDH | Abcam ab181602 | 1: 10000 | 36 |