Fig. 6

Analysis of clonal evolutionary trajectories in ctDNA from patient 45. A Clinical timeline for patient 45 showing treatment dates for primary tumour resection, adjuvant chemotherapy (gemcitabine) and sampling timepoints, as days from initial diagnosis. B Scatterplot showing the estimated prevalence of inferred clones in ctDNA, across sampled timepoints. C Longitudinally observed phylogenetic tree showing the predicted evolutionary trajectories of individual ctDNA clones. Coloured triangles represent mutations unique to each respective clone. Examples of unique driver mutations acquired in individual clones are shown on the tree. D Clonal diagram of the tree structure from (D) showing differences between estimated clonal proportions across sampled timepoints. GEM, Gemcitabine