Innovative mouse models for the tumor suppressor activity of Protocadherin-10 isoforms

BMC Cancer

Table 1 Origin and characteristics of pinnal-tumor derived (PTD) cell lines and rescued derivatives thereof

Mouse tag number	Genotype of original mouse				PTD cell line	Origin	Enrichment	Colonies in soft agar	In vitro invasion	Growth rate of s.c. allograft
Mouse tag number	GFAP-Cre	Pcdh10 all	p53	Rb1	PTD cell line	Origin	Enrichment	Colonies in soft agar	In vitro invasion	Growth rate of s.c. allograft
9034	tg/+	fl/fl	fl/fl	fl/fl	PTD4	Pinnal tumor, dissociated overnight	pool	++	+++	quick
~					PTD7	~	subclone	++	+++	quick
~					PTD8	~	subclone	++	+++	intermediate
8822	tg/+	fl/fl	fl/fl	+/+	PTD11	Pinnal tumor, 4 h dissociation	subclone	none	+	slow
~					PTD12	~	subclone	none	+	very slow
~					PTD12AD4	PTD12 allograft	pool	n.d.	n.d.	quick
9135	tg/+	fl/fl	fl/fl	+/+	PTD17	Pinnal tumor, 1 h dissociation	subclone	none	n.d.	intermediate
~					PTD19	Pinnal tumor, 4 h dissociation	subclone	none	n.d.	intermediate
1729	tg/+	fl/fl	fl/fl	fl/fl	PTD24	Middle part of pinnal tumor, 1 h dissociation	pool	++	n.d.	very quick
~					PTD25	Outer part of pinnal tumor, 1 h dissociation	pool	+++	+++	very quick
9988	tg/+	fl/fl	fl/fl	fl/+	PTD26	Pinnal tumor, 2 h dissociation	pool	n.d.	++	intermediate
~					PTD27	Pinnal tumor, 4 h dissociation	pool	n.d.	+	intermediate
Pcdh10 Rescued Derivatives
9034	tg/+	fl/fl	fl/fl	fl/fl	PTD7_RS	PTD7, rescued with Pcdh10 short isoform 1	FACS sorted	none	-(+)	reduced rate
~					PTD7_RL	PTD7, rescued with Pcdh10 long isoform 4	FACS sorted	none	-(+)	reduced rate
1729	tg/+	fl/fl	fl/fl	fl/fl	PTD25_RS	PTD25, rescued with Pcdh10 short isoform 1	FACS sorted	none	–	no tumor
~					PTD25_RL	PTD25, rescued with Pcdh10 long isoform 4	FACS sorted	none	–	reduced rate

ISSN: 1471-2407