Fig. 4

Pcdh10 expression levels in Pcdh10long^−/− mice. A-D Western blot analysis of brain lysates from wild-type (+/+), heterozygous (+/−) and homozygous (−/−) mutant Pcdh10long mice. See Additional file 8: Table S7 for antibody information. Loading control: β-tubulin. The corresponding original, uncropped Western blots are shown in Additional files 29 to 32. A Monoclonal antibody 5G10 detects only long isoforms. B Monoclonal antibody 2H8 detects all isoforms. C Polyclonal antibody G415 detects only long isoforms. D Polyclonal antibody G418 detects only the short isoform. Arrows point to the band corresponding to the long isoforms, arrowheads point to the band corresponding to the short isoform. Asterisks indicate aspecific bands for the polyclonal antibodies. E qRT-PCR analysis of brains from wild-type, heterozygous and homozygous mutant Pcdh10long mice. Expression levels of all (black bars), long (grey bars) and short (white bars) isoforms were determined in 3 mouse brains of each genotype and data are represented as Mean + SD. Values for wild-type brains were set to 1. See Additional file 6: Table S5 for primer information