Fig. 12
From: Innovative mouse models for the tumor suppressor activity of Protocadherin-10 isoforms
Restored expression of Pcdh10 isoforms reduces malignancy of pinnal-tumor derived cell lines PTD7 and PTD25. A Immunofluorescent detection of ectopic expression of Pcdh10 (long isoform 4) at cell-cell contacts (arrows) in transduced and FACS sorted PTD7_RL cultures, using MAb 5G10 as primary antibody specific for long Pcdh10 isoforms (Additional file 8: Table S7). Scale bar: 20 μm. B Immunofluorescent detection of ectopic expression of HA-tagged Pcdh10_short isoform 1 at cell-cell contacts (arrows) in transduced and FACS sorted PTD25_RS cultures, using anti-HA as primary antibody. Scale bar: 20 μm. C,D Allograft formation after s.c. injection of single-cell suspensions. Tumor volumes are the average + SD for 4 to 5 injected mice. See Additional files 18 and 22: Tables S12 and S14 for quantitation and statistical analysis. C Inoculation of PTD7 cells and their Pcdh10 rescued derivatives. PTD7_RS expresses ectopic Pcdh10_short isoform 1; PTD7_RL expresses the long isoform 4. The number of inoculated cells was 106 and 105 as indicated. D Inoculation of PTD25 cells and their Pcdh10 rescued derivatives. PTD25_RS expresses ectopic Pcdh10_short isoform 1; PTD25_RL expresses the long isoform 4. The number of inoculated cells was 105 and 104 as indicated