Fig. 10

Invasive growth in 3D collagen matrices by spheroids generated from PTD cell lines. A Quantitation of representative experiments is shown. For details on PTDs see Table 1. Genotypes of the original pinnal-tumor bearing mice were: GFAP-Cre^tg/+;Pcdh10all^fl/fl;p53^fl/fl;Rb^fl/fl (for PTD4, 7, 8, 25), GFAP-Cre^tg/+;Pcdh10all^fl/fl;p53^fl/fl;Rb^fl/+ (for PTD26, 27) and GFAP-Cre^tg/+;Pcdh10all^fl/fl;p53^fl/fl;Rb^+/+ (for PTD11, 12). Sphere perimeters at 48 and 92 h were normalized to the sphere perimeter at t0 (fold increase). MNNG-HOS is a human cancer cell line, which served as a positive control [62]; The negative control is a non-invasive human breast cancer cell line T47D (sphere diameter fold increase ~ 1). For each cell line, the number of spheroids observed is given between brackets, and the values plotted are the means + SEM. B Representative data for three time points are shown, up to 5 days after seeding (T5d). PTD7 and PTD25 are the original PTD cell lines obtained from mice with GFAP-Cre^tg/+;Pcdh10all^fl/fl;p53^fl/fl;Rb^fl/fl genotype. PTD7_RS and PTD25_RS are transduced and FACS-sorted derivative cell populations of, respectively, PTD7 and PTD25, rescued for Pcdh10 ablation by cDNA expression of the Pcdh10_short isoform 1 (see text, Table 1 and Additional file 18: Table S12). PTD7_RL and PTD25_RL are similar derivative cell populations, but rescued by cDNA expression of the Pcdh10_long isoform-4. Note the strongly reduced invasion or even lack of invasion upon Pcdh10 rescue. Scale bars, 150 μm