Fig. 7

ZFP423 overexpression (OE) promotes adipocyte differentiation in DDLPS Lipo224. A Oil Red O staining of well-differentiated liposarcoma (WDLPS) and DDLPS (Lipo224) after 10 days of adipogenic induction with accompanying quantification of Oil Red O staining. B Gene expression of factors involved in adipogenesis at pre-induction (D0) and day 10 (D10). C Western blot analysis of ZFP521, SIAH2, PPARG, ZFP423, and β-actin at pre-induction (0) and day 10 [10] post-induction. Bar plots depict mean ± standard deviation compared to pre-induced HuASC; *, p < 0.05, **, p < 0.01, ***, p < 0.001. Each point in the plots represents a technical replicate. 224, Lipo224. Full-length western blot replicates and densitometry ratio quantifications are shown in Supplemental Fig. S4. Note that the western blots for PPARG1 and PPARG2 detection in S4 (native MW around 50 kD) include very high molecular weight bands that may represent ubiquitin-modified PPARG