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Fig. 6 | BMC Cancer

Fig. 6

From: Expression of the preadipocyte marker ZFP423 is dysregulated between well-differentiated and dedifferentiated liposarcoma

Fig. 6

ZFP423 knockdown attenuates adipocyte differentiation in human ASC and DDLPS Lipo863 cells. A Oil Red O staining of human retroperitoneal adipose primary cells (HuASC) and DDLPS (Lipo863) after 4 days of adipogenic induction with accompanying quantification of Oil Red O staining. B Gene expression of factors involved in adipogenesis at pre-induction (D0) and day 4 (D4) post-induction. C Western blot analysis of ZFP521, SIAH2, PPARG, ZFP423, and β-actin at pre-induction (0) and day 4 [4] post induction. Bar plots depict mean ± standard deviation compared to pre-induced HuASC; *, p < 0.05, **, p < 0.01, ***, p < 0.001. Each point in the plots (A and B) represents a technical replicate. Full-length western blot replicates and densitometry ratio quantifications are shown in Supplemental Fig. S3. Note that the western blots for PPARG1 and PPARG2 detection in S3 (native MW around 50 kD) include very high molecular weight bands that may represent ubiquitin-modified PPARG

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