Fig. 5

ZFP423 protein expression is associated with upregulation of PPARG2 protein in normal adipose tissue primary cells (HuASC) and DDLPS cell lines. A Oil Red O staining of human retroperitoneal adipose primary cell (HuASC), WDLPS (WD), and DDLPS (224, 863, and 815) after 14 days of adipogenic induction. B Quantification of Oil Red O staining. C Western blot analysis of ZFP521, SIAH2, PPARG, and β-actin from HuASC, WDLPS, and DDLPS cell lines at pre-induction (Day 0), and 3 (Day 3) or 14 days (Day 14) post induction. 224, Lipo224 cell line; 863, Lipo863 cell line; 815, Lipo815 cell line. A full-length, original and unprocessed version of the western blot for each antibody and densitometry ratio quantification is shown in Supplemental Fig. S2. The plots are representative of experiments that were repeated at least twice. Bar plots depict mean ± standard deviation compared to HuASC; *, P < 0.05, ** P < 0.1 and *** P < 0.001