Fig. 1

Adipogenesis in LPS tissues is dysregulated at preadipocyte commitment. A Demographic information available for tissues included in the analysis. B Western blots analysis of SIAH2, ZFP423, PPARG, and β-actin. Biological replicates of western blot analyses are available in Supplemental Fig. S1, along with full-length original western blots and densitometry ratio quantifications. C Gene expression of markers of mature adipocytes (PPARG2, ADIPOQ, PLIN1), preadipocyte markers (PREF-1, PDGFRA, ZFP423) or regulatory factors involved in adipogenesis (CEBPB, CEBPD, ZFP521, SIAH2). D Gene expression of macrophage (CD11B, CD64) and cytokine (IL6, IL10) markers. Each point in bar plot represents a single technical replicate from frozen normal human retroperitoneal adipose tissues (rpWAT, n = 3), well-differentiated liposarcoma (WD, n = 2), and dedifferentiated (DD, n = 2). Bar plot represents mean ± standard deviation of fold change when compared to rpWAT; *, p < 0.05; **, p < 0.001; ***, p < 0.0001. N/A, not available; UD, undetermined; AA/Black, African American/Black; W, White