Fig. 4

PARPi induce apoptosis in BLCA cells in vitro. A The BLCA cell lines UM-UC-3 and T-24 and the normal urothelial cells SV-HUC-1 were treated with sub-IC₅₀ concentrations of niraparib, olaparib, rucaparib (5 μM each), talazoparib (0.5 μM) either singly or in combination with sub-IC₅₀ concentration of cisplatin (0.5 μM) for 72 h. The resulting cell lysates were subjected to Western blotting with antibodies against the apoptotic markers cleaved/whole caspase 3, cleaved/whole caspase 9, or cleaved/whole PARP. Representative images of 3 independent experiments with duplicates are presented. B Relative band intensities (compared to whole caspase/PARP and Tubulin) were measured using ImageJ and are presented for Cleaved caspases 3 and 9 and cleaved PARP