Fig. 5
From: NAP1L1 promotes tumor proliferation through HDGF/C-JUN signaling in ovarian cancer
Transfecting HDGF reverses the suppressive effects in OC cells. A Transfecting HDGF reverses cell proliferation identified using CCK-8 assay in OC cells. B-C Transfecting HDGF reverses cell proliferation identified using EdU assay (scale bar, 50 μm). D-E Flow cytometric analysis indicates transfecting HDGF reverses cell cycle distribution. F The efficiency of the transiently transfected HDGF plasmid into the NAP1L1 knock down cells was examined by Western blotting. In those NAP1L1 silenced cells, we observed a decrease in the expression of C-JUN and CCND1, with the overexpressing of HDGF, the C-Jun and CCND1 protein expression also increased. *p < 0.05, **p < 0.01, and ***p < 0.001