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Fig. 3 | BMC Cancer

Fig. 3

From: NAP1L1 promotes tumor proliferation through HDGF/C-JUN signaling in ovarian cancer

Fig. 3

NAP1L1 suppressed the proliferation of OC cells (instantaneous and stable interference). A-B The expression of NAP1L1 in OC cell lines was examined by RT-qPCR and Western blotting to verify the efficiency of interference following instantaneous infection of siNAP1L1 and (G) lentiviral infection of shNAP1L1. C-D EdU assays (scale bar, 50 μm) were conducted after transfection with siNAP1L1#1 or #2. The first segment was selected for subsequent experiments due to the higher efficiency. EF MTT assays were conducted after transfection with siNAP1L1#1. H Colony numbers of shNAP1L1 and shNC. I-J Cell cycle distribution of siNAP1L1/siNC in OC cells and statistical analysis of cell percentages in G1, S, G2 in the above cells. K-L The apoptosis of the above cells was analyzed by flow cytometry. *p < 0.05, **p < 0.01, and ***p < 0.001

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