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Fig. 4 | BMC Cancer

Fig. 4

From: Norepinephrine promotes glioma cell migration through up-regulating the expression of Twist1

Fig. 4

Twist1 overexpression facilitated mesenchymal-like phenotype and migration of glioma cells. Lentiviruses were used to establish the stable glioma cell lines with Twist1 overexpression. The levels of Twist1, mesenchymal markers and the migration ability were measured in the indicated cells. A The overexpression of Twist1 in established U251 cell lines were validated by real-time PCR (left panel, n = 3, p = 0.038) and western blot (right panel). Student’s t-test was used for statistical analysis. B The overexpression of Twist1 in established LN229 cell lines were validated by real-time PCR (left panel, n = 3, p = 0.005) and western blot (right panel). Student’s t-test was used for statistical analysis. C Real-time PCR revealed the increased mRNA levels of the mesenchymal markers in U251/LN229 LVTwist1 cells (U251: n = 3, N-cadherin p = 0.016, Fibronectin1 p = 0.014, Vimentin p = 0.021; LN229: n = 3, N-cadherin p = 0.011, Fibronectin1 p = 0.037, Vimentin p = 0.008). Student’s t-test was used for statistical analysis. D The higher expressed mesenchymal markers in LVTwist1 cells were shown. The relative levels of those markers were noted as the mean number underlying the band images. E Twist1 overexpression led to higher travelled distances of wound healing both in U251 (n = 5, p = 0.018) and LN229 cells (n = 5, p = 0.002). Scale bar = 50 μm. Student’s t-test was used for statistical analysis. *p < 0.05, **p < 0.01

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