Fig. 5

Circ_0064288 increases ROCK1 expression by targeting miR-335-5p. A. Three bioinformatics algorithms (miRDIP, StarBase and Targetscan) were used to predict the binding sites of miR-335-5p within the 3’UTR of ROCK1. B. Dual-luciferase reporter experiment was executed to confirm the predicted binding sequence between miR-335-5p and ROCK1 3’UTR. C. StarBase database was utilized to analyze ROCK1 expression in HCC tissues and paracancerous tissues. D-F. qRT-PCR and Western blot experiments were applied to detect ROCK1 expression in HCC cells after the expression of circ_0064288 and miR-335-5p were selectively regulated. **P < 0.01, ***P < 0.001, ns, means no statistical significance