Fig. 4

Overexpression of miR-335-5p counteracted the biological effects of circ_0064288 on HCC cells. A. Huh7 cells overexpressing circ_0064288 were transfected with miR-335-5p mimics, and the expression level of miR-335-5p in HCC cells was detected by qRT-PCR. B. CCK-8 assay was used to detect the proliferation of HCC cells after the transfection. C-D. Transwell assay (scale bar, 25 μm) and wound healing assay (scale bar, 100 μm) were used to detect the migration of HCC cells after the transfection. *P < 0.05, **P < 0.01, and ***P < 0.001