Fig. 3

ZNF703 regulates cell cycle of TNBC. A, B Inhibiting ZNF703 induced G1-phase arrest in BT-549 and MDA-MB-468 cell lines. Cells were treated with NC or ZNF703-siRNA for 72 h, and DNA contents were detected and analyzed by flow cytometry assay. The percentage of cells in G1, S and G2/M of cell cycle were calculated. These results were from one representative experiment of three independent experiments. C Immunoblotting (IB) of lysates of BT-549 NC, BT-549 siRNA, MDA-MB-468 NC and MDA-MB-468 siRNA cells using the indicated antibodies. A HSP90α antibody was used as a loading control. The experiment was repeated for three times and one representative result was shown