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Table 3 Primers for Real-time PCR and siRNA/shRNA

From: Tenascin-C promotes bladder cancer progression and its action depends on syndecan-4 and involves NF-κB signaling activation

Gene ID Gene Primers
NM_001145138.1 P65 F ACG AAT GAC AGA GGC GTG TAT AAG G
R CAG AGC TGC TTG GCG GAT TAG
NM_002046.4 GAPDH F AAC AGC GAC ACC CAT CCT C
R CAT ACC AGG AAA TGA GCT TGA CAA
NM_004360.3 E-Cadherin F TGC CCA GAA AAT GAA AAA GG
R GTG TAT GTG GCA ATG CGT TC
NM_001792.3 N-Cadherin F ACA GTG GCC ACC TAC AAA GG
R CCG AGA TGG GGT TGA TAA TG
NM_003380.3 Vimentin F GAG AAC TTT GCC GTT GAA GC
R GCT TCC TGT AGG TGG CAA TC
NM_005985.3 Snail1 F ACC CCA ATC GGA AGC CTA ACT
R GGT CGT AGG GCT GCT GGA A
NM_004530.4 MMP2 F CTC ATC GCA GAT GCC TGG AA
R TTC AGG TAA TAG GCA CCC TTG AAG A
NM_004994.2 MMP9 F TGA CAG CGA CAA GAA GTG
R CAG TGA AGC GGT ACA TAG G
NM_002999.3 Syndecan-4 F CCA GTT TGA TGT TGC TGG GTG GTT
R AGC CCT AGA GCC TGA AGA AAG CAA
Syndecan-4 siRNA Si 5 -AAG GCC GAT ACT TCT CCG GAG-3
Sc 5 -AAG GCT CTC CGG AGC GATA CT-3
NM_002160.3 Tenascin-C F AGC TTC CAA GAA ACA CCA CTT C
R CCA TCC CAG CCA ACC TCA
Tenascin-C shRNA F 5′-CAC CGC ACC TGA AGG CCT GAA ATT CTT CAA GAG AGA ATT TCA GGC CTT CAG GTG CTT TTT TG-3’
R 5′-GAT CCA AAA AAG CAC CTG AAG GCC TGA AAT TCT CTC TTG AAG AAT TTC AGG CCT TCA GGT GC-3’