Fig. 6
TN-C contributes to activation of NF-κB signaling depends on membrane receptor syndecan-4. A Western blot indicates the ubiquitous expression of syndecan-4 (syn-4) in parental BCa cell lines, GAPDH was a loading control; B Western blot indicates that there is no visible discrepancy expression of syn-4 in T24sc Vs T24siTN-C and 5637Vec Vs 5637TN-C, suggesting that TN-C has no effect on the syn-4 expression in tumor cell line, and siRNA to knock down syn-4 significantly attenuated expression of syn-4 in both cell lines, indicating the perfect efficiency; C Quantification of Boyden chamber assay indicates knocking down syn-4 in both cell lines decreases the ability of migration and invasion in the presence of TN-C, *P < 0.05; D Immunofluorescent staining indicates that TN-C induced nuclear translocation of NF-κB is diminished in the absent of syn-4, Bar: 20 μm