Fig. 2

TN-C expression in BCa cell lines. A Western blot of TN-C expression in parental BCa cell lines; GAPDH was a loading control, indicating different expression profile, with the strongest expression for T24 and J82, accompanied by the weakest for 253 J and 5637; B Real time PCR indicates TN-C expression in parental BCa cell lines, P < 0.05, suggesting expression differences; C ELISA depicting TN-C in parental BCa cell line medium, indicating TN-C also as a component of extracellular matrix (P < 0.05). This expression pattern got consistent with protein and mRNA indicated by Western Blot and real time PCR; D Western blot indicates efficiency of TN-C knockdown (T24^{si TN-C}Vs T24^sc and J82^{si TN-C} Vs J82^sc) or TN-C over-expression (5637^TN-CVs 5637^Vec and 253J^TN-CVs 253J^Vec), GAPDH was a loading control; E Real time PCR indicates the efficiency of TN-C knockout (T24^{si TN-C}Vs T24^sc and J82^{si TN-C} Vs J82^sc) or TN-C over-expression (5637^TN-CVs 5637^Vec and 253J^TN-CVs 253J^Vec), *P < 0.05; F ELISA data depicting TN-C in medium from cells with TN-C knocked out or with TN-C stably overexpressed, *P < 0.05