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Fig. 6 | BMC Cancer

Fig. 6

From: Loss of REST in breast cancer promotes tumor progression through estrogen sensitization, MMP24 and CEMIP overexpression

Fig. 6

Semi-quantitative ChIP-PCR analysis of RE1 sites located with the MMP24 gene. A MMP24 gene locus showing REST binding sites (RE1 sites) and their locations relative to the transcription start site (TSS, + 1). B DNA immunoprecipitation analysis in MCF-7 cells (control vs siREST). ChIP using total H3 (positive control), REST, RNA polymerase II, H3K27Me3, and rabbit IgG (negative control) antibodies at RE1 Site 1, Site 2 and Site 3. A representative experiment of three independent biological replicates is shown. Gels were cropped for clarity. Relative densities (n = 3) were quantified in ImageJ and normalized to the respective inputs. Error bars indicate ± SD

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