Fig. 4

Breast cancer cell lines showing REST and REST target gene expression. A Western blot of REST and CEMIP expression in breast cancer cell lines MDA-MB-468, T-47D, HCC1937 + , HCC1937 -, HCC1937, MCF-7, MCF-7 with REST knockdown, MDA-MB-231, and MDA-MB-231 with REST knockdown. Beta-actin was used as a loading control. B REST knockdown using siRNA leads to a significant upregulation in CEMIP and MMP24 expression in MCF-7 cells. C Western blot showing overexpression of CEMIP at 24 h and 48 h and decreased REST expression upon REST knockdown using siRNA. Densities for CEMIP and REST were calculated using ImageJ and normalized to beta-actin. D qPCR data showing a significant reduction in REST expression for MDA-MB-231 cells. CEMIP and MMP24 were significantly upregulated in MDA-MB-231 cells treated with siRNA targeting REST. Western blots have been cropped for clarity. Error bars indicate ± SD, Unpaired T-test was performed, *P < 0.05, **P < 0.01