Fig. 1
Inoculation of LNCaP cells into the right dorsal lobe (red arrow) of the prostate of male Balb/c mice and a schematic of the experimental design. A The trigonal bipyramidal dorsal lobe of the prostate was located. B A needle attached to a Hamilton syringe was inserted into the right dorsal lobe, approximately parallel to the long axis of the dorsal lobe. C A 20μL suspension containing 1 × 106 LNCaP cells was slowly injected. D A small blister was observed after the needle was retracted. E Schematic of the experimental design. Twenty-two male Balb/c athymic nude mice (5 weeks old) were acclimated for 3 weeks, and orthotopic injection was performed at Week 8. Starting 2 weeks post-injection, tumor growth was monitored by ultrasound every 5 days. Mice were sacrificed at Week 18, and tumors were excised for histologic analysis. SV, seminal vesicle; R, rectum