Fig. 9

Detection of HPVE6, and E7 transcripts in exosomal RNA of cervical cancer cells undergoing genotoxic stress. Gel images showing E6 and E7-specific amplification in cDNA prepared from exosomal RNA of 5-FU or cisplatin-treated SiHa (A) and HeLa cells (B). RT-PCR was performed on exosomal RNA (2 μg/reaction). Cellular RNA from corresponding untreated cells was used as a reference. C33a cDNA was used as a negative control. Exo.-exosomal cDNA, M-marker, Neg: template free reaction, PD-primer dimer