Fig. 4
Role of Rac/CDC42 and MAPK pathways in the regulation of CK2α genes induced by OGR1: Inhibition of Rac activity abrogated up-regulation of CSNK2A3 induced by OGR1. A549 cells were co-transfected with OGR1 or pcDNA3.1 (Vector) and negative dominant mutants of Rac (pcDNA3.1-RacT17N) or negative dominant mutants of CDC42 (pcDNA3.1-cdcT17N). A Semi-quantitative RT-PCR was performed to analyze CSNK2A1 and CSNK2A3 transcript expression. β-actin was used as a control for equal loading. B Western blot assay was further analyzed to check the protein expression of CK2α. MAPK pathways in the regulation of CK2α genes induced by OGR1. A549 cells were also transfected with pDNA3.1-OGR1 or pcDNA3.1 (Vector) and activation of MAPK families, C ERK, JNK, p38, were analyzed by western blotting. D and E Semi-quantitative RT-PCR and western blot were performed to analyze CSNK2A3 transcript and CK2αP expression, respectively, in the presence of specific MAPK inhibitors. β-actin was used as a control for equal loading. The display of cropped gels and blots are to improve the clarity and conciseness of the presentation. However, full length gels and blots are also included in supplementary file 2. Error bars represent the Mean ± SD of three independent experiments. The p-value of statistical significance was set as p < 0.05 (*)