Skip to main content
Fig. 2 | BMC Cancer

Fig. 2

From: Ovarian cancer G protein-coupled receptor 1 inhibits A549 cells migration through casein kinase 2α intronless gene and neutral endopeptidase

Fig. 2

CK2 is upstream of NEP in the OGR1 signaling pathway: Western blot assay of NEP and CK2αP expression in presence or absence of specific inhibitors. A A549 cells were transfected with pDNA3.1-OGR1 (OGR1) or pcDNA3.1 (Vector) and NEP expression was analyzed with anti-NEP in the presence or absence of CX-4945, CK2 inhibitor (CX4945), and B protein band intensities were analyzed by using ImageJ software, and represented graphically after normalization to actin. C Expression of CK2α was analyzed with anti CK2α in the presence or absence of NEP inhibitor, thiorphan (THP), and D protein band intensities were analyzed by using ImageJ software and represented graphically after normalization to actin. E Semi-qPCR showing the expression of NEP and CK2 in the presence or absence of Gαi inhibitor PTX and F intensities of DNA bands were analyzed by using ImageJ software and represented graphically. The display of cropped gels and blots are to improve the clarity and conciseness of the presentation. However, full length gels and blots are also included in supplementary 2. Replicates of NEP and actin blots are also included in supplementary file 2. Error bars represent the Mean ± SD of three independent experiments. The p-value of statistical significance was set as p < 0.05 (*)

Back to article page