Table 1 Primer sequences for functional analysis of S100 gene family members used in this study

RT-PCR (mRNA)

S100A1

Forward

5'-ATGGGCTCTGAGCTGGAGAC-3'

285 bp

Reverse

5'-TCAACTGTTCTCCCAGAAGAAAT-3'

S100A2

Forward

5'-ATGATGTGCAGTTCTCTGGAGC-3'

297 bp

Reverse

5'-TCAGGGTCGGTCTGGGCAGC-3'

S100A3

Forward

5'-AGTGAGGATGGCCAGGCCTCTG-3'

323 bp

Reverse

5'-GGAGCAGAGGCTACTGGGAGCA-3'

S100A4

Forward

5'-GCCACCATGGCGTGCCCTCTGGAGAA-3'

306 bp

Reverse

5'-TCATTTCTTCCTGGGCTGCTTATCTG-3'

S100A5

Forward

5'-ACACTGTGATGGAGACTCCTCT-3'

287bp

Reverse

5'-TCACTTGTTGTCCTCTAGAAAGAAGT-3'

S100A6

Forward

5'-ATGGCATGCCCCCTGGATCAG-3'

273bp

Reverse

5'-TCAGCCCTTGAGGGCTTCATT-3'

S100A7

Forward

5'-ATGAGCAACACTCAAGCTGAGAGGT-3'

306 bp

Reverse

5'-TCACTGGCTGCCCCCGGAACAG-3'

S100A8

Forward

5'-ATGTTGACCGAGCTGGAGAAAG-3'

282 bp

Reverse

5'-CTACTCTTTGTGGCTTTCTTCATGG-3'

S100A9

Forward

5'-ATGACTTGCAAAATGTCGCAGCTGG-3'

350 bp

Reverse

5'-TGGTCTTAGGGGGTGCCCTCC-3'

S100A10

Forward

5'-CACCAAAATGCCATCTCAAATGG-3'

303 bp

Reverse

5'-GCCTACTTCTTTCCCTTCTGCT-3'

S100A11

Forward

5'-ATGGCAAAAATCTCCAGCCCTACAG-3'

318 bp

Reverse

5'-TCAGGTCCGCTTCGGGAAGGG-3'

S100A12

Forward

5'-ATGACAAAACTTGAAGAGCATCTGG-3'

279bp

Reverse

5'-CTACTCTTTGTGGGTGTGGTAATGG-3'

S100A13

Forward

5'-ATGGCAGCAGAACCACTGAC-3'

297 bp

Reverse

5'-TTACTTCTTCCTGATCTTCAGGTCT-3'

S100B

Forward

5'-ATGTCTGAGCTGGAGAAGGC-3'

279 bp

Reverse

5'-TCACTCATGTTCAAAGAACTCGTGG-3'

CALB3(S100G)

Forward

5'-ATGAGTACTAAAAAGTCTCCTGAGGA-3'

256 bp

Reverse

5'-TATTTTGTTTTCTCCTTCACTGGGA-3'

S100P

Forward

5'-ATGACGGAACTAGAGACAGCCAT-3'

288 bp

Reverse

5'-TCATTTGAGTCCTGCCTTCTCAAAG-3'

FLAG-tagged cDNA

S100A1

Forward

5'-CGCAAATGGGCGGTAGGCGTG-3'

Reverse

5'-GAACTGTTCTCCCAGAAGAA-3'

shRNA

S100A1

Forward

Reverse