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Fig. 5 | BMC Cancer

Fig. 5

From: Functional interaction between S100A1 and MDM2 may modulate p53 signaling in normal and malignant endometrial cells

Fig. 5

Changes in migration following overexpression or knockdown of S100A1 in Hec6 and Hec251 cells. A, C Upper: a scratch was made in the middle of a layer of confluent H6-S100A1 (A) or H251-shS100A1 cells (C), and phase contrast images were taken 72 h or 96 h later. The red lines indicate borderlines between confluent cell layers and wound area. Lower: the wound areas were calculated using Image J software version 1.41, with the area at 0 h post-wounding set as 1. The experiments were performed in triplicate. Data are expressed as mean ± SDs. B Upper panel: stable H6-S100A1 cells and control cells were seeded in a 24-well transwell plate and incubated for 48 h in serum-free medium. Cells that migrated were stained with hematoxylin-eosin and counted using a light microscope. Lower panel: cell numbers are presented. The experiment was performed in duplicate. Con, control

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