Fig. 4

Changes in apoptosis following overexpression or knockdown of S100A1 in Hec6 or Hec251 cells. A, D Left: stable H6-S100A1 (A), H251-shS100A1 cells (D), and controls were treated with 1 μg/mL Adriamycin (ADR) for the time shown. Daggers indicate sub-G1 fraction. Right: the percentages of cells undergoing apoptosis (sub-G1 fractions) were calculated following flow cytometry. C, control (B, E) Left: after 1 μg/mL ADR treatment, stable H6-S100A1 (B), H251-shS100A1 cells (E), and controls undergoing apoptosis are indicated by arrows. Original magnification, x400. Right: the numbers of apoptotic cells are shown as mean ± SDs. Con, control (C, F) Western blot analysis of the indicated proteins in total lysates from stable H6-S100A1 cells (C), H251-shS100A1 cells (F), and controls treated with 1 μg/mL ADR for the times shown