Fig. 1

Interactions between S100A1, MDM2, and p53 in Hec6 cells. A Western blot analysis (WB) with anti-MDM2 (upper panel), anti-p53 (middle panel), and anti-FLAG M2 antibodies (lower panel) after immunoprecipitation (IP) with the indicated antibodies using stable H6-S100A1#55 cell lysates. Input represents 5% of the total cell extract. Normal rabbit IgG was used as a negative control. In the middle panel (p53), the band indicated by an arrow in the S100A1 lane is non-specific, since the molecular weight is slightly higher than that of endogenous p53 (input). B Western blot analysis of the indicated proteins in H6-S100A1 cells in response to Nutlin-3A treatment for the times shown. The p53 and MDM2 bands are indicated by arrows