Fig. 6

SOX4 was a downstream gene for miR-873-5p in ESCC cells. A The common target genes in TargetScan and GEPIA ESCA were sought by Venn Diagram analysis. B, C GEPIA database showed the expression of SOX4 (B) and ENAH (C) in ESCA samples. D SOX4 and ENAH mRNA levels were detected by RT-qPCR in EC9706 and KYSE30 cells transfected with miR-NC or miR-873-5p. E The combined target sites of SOX4 3′UTR for miR-873-5p were shown by TargetScan. F The measurement of luciferase activity was performed by the dual-luciferase reporter system after co-transfection of SOX4 3′UTR-WT or SOX4 3′UTR-MUT and miR-873-5p or miR-NC. G SOX4 protein expression was examined applying with western blot in miR-NC or miR-873-5p transfection group. *P < 0.05