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Table 2 Summary of time points of the translational program. h = hour

From: The ISCON-trial protocol: laparoscopic ischemic conditioning prior to esophagectomy in patients with esophageal cancer and arterial calcifications

  ICG fluorescence Biomarkers
< 24 h to start of ISCON   Biopsies of anastomotic site + peripheral blood (10 ml EDTA)
ISCON: before occlusion of arteries X  
ISCON: 10 min after occlusion of arteries X  
< 24 h to start of esophagectomy   Biopsies of anastomotic site + peripheral blood (10 ml EDTA)
Esophagectomy: before formation of gastric tube X  
Optional: esophagectomy: before formation of intrathoracic anastomosis (X)  
Esophagectomy: after completion of anastomosis   Gastric anastomotic donut or tip of gastric tube