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Fig. 2 | BMC Cancer

Fig. 2

From: SYT-SSX1 enhances the invasiveness and maintains stem-like cell properties in synovial sarcoma via induction of TGF-β1/Smad signaling

Fig. 2

SYT-SSX1 enhances stem-like cell properties. A The number of cells, volume, and sphere-forming rates were calculated for SYT-SSX1 expressing cells and parental SW982 control cells. B Transwell assay showed that the migration ability of SYT-SSX1 sphere cells was stronger than that of SW982. C-D Enhanced migration ability of SYT-SSX1 sphere cells. Results from microscopy indicate the observations in the scratch experiment at 0, 24, and 48 h (Magnification: 100X). The scratch area was quantified. E-F qPCR and western blotting showed that cancer stem cell (CSC) markers (SOX-2, Nanog, and OCT-4), self-renewal factors (LIF and LIFR), and proliferation index (Ki-67) were highly expressed in SYT-SSX1 sphere cells compared with that of SW982. G Flow cytometry showed SYT-SSX1sp cells highly expressed the CSC marker CD133; the protein expression level was significantly higher than SW982 sphere cells. H Fifteen cases of synovial sarcoma tissue and 8 cases of next to the tumor (control group) were examined using qRT-PCR to detect the expression of CSC markers, self-renewal factors, and proliferation index. All experiments were repeated at least three times. Error bars represent the means ± SD. β-actin served as an internal control. *P < 0.05, **P < 0.01, ***P < 0.001

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