Fig. 4

Over-expression of PCBP1 abolishes the effects of C12orf48 in gastric cancer cells. HGC27 gastric cancer cells were transfected with control, C12orf48 over-expression vector or concurrent C12orf48 and PCBP1 over-expression vectors. A Proliferated cells were subjected to CCK-8 assays to measure O.D. values at 0, 24, 48, 72, 96 h after transfection. B 72 h after transfection, cellular apoptosis was detected with Annexin V-PE and 7-AAD using flow cytometry. C The rates of apoptosis cells were analyzed. D-G 12 h after transfection, cells were seeded into transwell inserts or chambers. By 60 h after transfection, representative images were captured from the migrated (D) and invaded (F) cells (magnification, × 100). And the numbers of migrated (E) and invaded (G) cells were counted and compared. H 96 h after transfection, the cells were subjected to western blotting. I The density of each band was measured and normalized to that of the respective Tubulin band. Experiments were repeated at least three times. Asterisks (*) indicate a significant difference compared with control groups (* P < 0.05, ** P < 0.01 and *** P < 0.001). The hash symbols (#) indicate a significant difference between specified groups (# P < 0.05, ## P < 0.01 and ### P < 0.001). NC, negative control. KD, knowdown. OE, overexpression