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Fig. 3 | BMC Cancer

Fig. 3

From: Depletion of C12orf48 inhibits gastric cancer growth and metastasis via up-regulating Poly r(C)-Binding Protein (PCBP) 1

Fig. 3

C12orf48 negatively relates to PCBP1 expression. AGS, BGC823 and HGC27 gastric cancer cell lines were transfected with control or C12orf48 shRNA 1 vector. A Cells were subjected to western blotting 96 h after transfection. B-C The density of each C12orf48 band (B) and each PCBP1 band (C) was measured and normalized to that of the respective Tubulin band. D GEPIA data showed the PCBP1 mRNA transcripts per million was comparable between gastric cancer tissues (n = 408) and non-neoplastic gastric epithelium tissues (n = 211). (E–F) Paired clinical gastric cancer tissues and non-neoplastic gastric epithelium tissues were immunohistochemically stained (n = 109). Representative images showed positive expression of PCBP1 protein in gastric cancer tissue (E), while negative expression in the normal one (F) (magnification, × 200). G Summary of PCBP1 expression in different gastric specimens. (H) Kaplan–Meier estimates estimated of the correlation between OS and PCBP1 expression. Experiments were repeated at least three times. Asterisks (*) indicate a significant difference compared with control groups (* P < 0.05, ** P < 0.01 and *** P < 0.001). NC, negative control. KD, knowdown

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