Fig. 2

Depletion of C12orf48 inhibited cellular proliferation, migration and invasion abilities, while facilitated apoptosis of gastric cancer cells. A Control and C12orf48 shRNA vectors were transfected into HEK-293 cells. The expression of C12orf48 mRNA was determined by qPCR. B AGS, BGC823 and HGC27 gastric cancer cell lines were transfected with control or C12orf48 shRNA 1 vector. The expression of C12orf48 mRNA was determined by qPCR. C-E AGS, BGC823 and HGC27 gastric cancer cell lines were transfected with C12orf48 shRNA 1 vector, over-expression vector or their respective controls. 60 h after transfection, proliferated cells were subjected to CCK-8 assays to measure O.D. (optical density) values. F-G 72 h after transfection, cellular apoptosis was detected with Annexin V-PE and 7-AAD using flow cytometry. And the rates of apoptosis cells were analyzed. H–K 12 h after transfection, cells were seeded into transwell inserts or chambers. By 60 h after transfection, the cells were imaged and counted (magnification, × 100). Representative images were captured from the migrated (I) and invaded (K) cells. The number of migrated (H) and invaded (J) cells were counted. Experiments were repeated at least three times. Asterisks (*) indicate a significant difference between knockdown group and control group (* P < 0.05, ** P < 0.01 and *** P < 0.001). The hash symbols (#) indicate a significant difference between overexpression group and control group (# P < 0.05, ## P < 0.01 and ### P < 0.001). NC, negative control. KD, knowdown. OE, overexpreesion