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Fig. 2 | BMC Cancer

Fig. 2

From: Quantitative proteomic analysis of GnRH agonist treated GBM cell line LN229 revealed regulatory proteins inhibiting cancer cell proliferation

Fig. 2

Expression of GnRH receptor in GBM cell line and tumor tissue samples. A RT-PCR analysis confirming expression of GnRH receptor (420 bp) (B) Western blot showing expression of GnRH receptor at 65 kDa, in LN229 cell line (C) Representative immunohistochemistry (IHC) image showing expression of GnRH receptor in GBM tumor tissue and non-tumor (epilepsy) tissue samples. IHC analysis showed the expression of GnRH receptor in 4 out of 10 GBM tissue samples. Epilepsy cases, used as non-tumor control, showed negative expression of GnRH receptor in astrocytic cells. Pituitary adenoma was used as a positive control. (Magnification- 10×). Full-length blot images are presented in Supplementary Fig. 1A and B. Full-length IHC images with a scale bar and magnification are presented in Supplementary Fig. 1E

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