Fig. 5

Polarization of macrophages can be facilitated by EVs from cervical cancer patients before and after radiotherapy. Peripheral blood mononuclear cells were obtained by Ficoll-Plaque density gradient centrifugation from healthy donors. Monocytes were isolated and cultured in RPMI 1640 medium. M2-polarized macrophages were obtained by IL-4+IL-13 stimulation for 48 hours. A, flow cytometry analysis the expression levels of cell surface and intracellular markers in macrophages before and after M2 polarization. M2 polarized macrophages were treated with EVs (the particle number per milliliter was 5 times higher than that in plasma) from cervical cancer patients before and after radiotherapy. B, flow cytometry analysis the expression levels of cell surface and intracellular markers. The dose effects of EVs on the expression levels of CD163 (C) and CCR7 (D) in M2 polarized macrophages were shown (n = 4). Bars presented as mean values of indicated markers. *, p < 0.05; **, p < 0.01. P value was calculated by two-tailed Mann Whitney U test