Table 1 Primers used for detection of human SMAD4 and mouse Smad4 transcripts
From: SMAD4–201 transcript as a putative biomarker in colorectal cancer
Transcript name (ID) | Forward and reverse primer sequence | Product length (bp) |
|---|---|---|
Human SMAD4–201 (ENST00000342988.8) | For: 5′-GCCCAGGTTATCCTGAATAC-3′ Rev.: 5′-GCTCAGACAGGCATCATTAC-3’ | 187 |
Human SMAD4–202 (ENST00000398417.6) | For: 5’-GAGAAGGAAGGTTATCCTG-3′ Rev.: 5′-CGTAATAGACATATTGTCC-3’ | 158 |
Total human SMAD4 | For: 5’-CACTACGAACGAGTTGTATCACC-3′ Rev.: 5′-CTTGATGGAGCATTACTCTGCAG-3’ | 71 |
Mouse Smad4–201 (ENSMUST00000025393.13) | For: 5’-GCCCAGGTCATCCTGCTCACC-3′ Rev.: 5′-GCTCAGACAGGCATCGTTAC-3’ | 188 |
Mouse Smad4–202 (ENSMUST00000114939.1) | For: 5’-CCTTGTGAAATGTGTTCTCATG-3′ Rev.: 5′-CCGACCAGCCACCTGAAGTCG-3’ | 429 |
Total mouse Smad4 | For: 5’-CGACTTCAGGTGGCTGGTCGG-3′ Rev.: 5′-GGATTCACACAGACACTGTCAC-3’ | 149 |