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Fig. 3 | BMC Cancer

Fig. 3

From: Overexpression of PFKFB3 promotes cell glycolysis and proliferation in renal cell carcinoma

Fig. 3

Knockdown of PFKFB3 inhibits glycolysis in RCC cells. (a) After shRNA transfection, Western blotting was employed as a validation measure for PFKFB3 knockdown in ACHN and A498 cell lines. (b) Protein levels of PFKFB3 were relatively quantified as the samples derive from the same experiment and that blots were processed in parallel. (c-d) Measurement of relative glucose uptake and lactate secretion in transfected cell lines. (e-f) ECAR levels were detected in transfected ACHN and A498 cells using an XF Extracellular Flux Analyzer. (g) Glycolysis (the ECAR increase following the addition of glucose) was further quantified by ECAR analysis. The results are presented as the mean ± SD. *, P < 0.05; **, P < 0.01

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