Fig. 9
From: Serotoninergic receptor ligands improve Tamoxifen effectiveness on breast cancer cells
Effect of SER79 and SER68 on Tamoxifen-resistant (MCF7-R) cells. a Estrogen starved MCF7-R were treated with SER79 (20 μM, 5 μM) or SER68 (20 μM) in presence of E2 (100 nM). b Estrogen-starved MCF7-R were treated with Tamoxifen (5 μM) in presence of E2 and SER79 (20 μM) or SER68 (20 μM). a,b As positive control, the cells were treated with E2 alone, in absence or in presence of SER (dotted line). After 72 h, cell viability was assessed by sulforhodamine B assay (see Methods). The results were reported as percentage of viable cells compared to positive control, considered as maximum viability (100%). c mRNA levels of CTGF were determined by qPCR (see Methods and Table 1) in MCF7-R cells upon treatment with SER68 (20 μM). Data were normalized on Ribosomal Protein S23 (Rps23) gene as internal standard and reported as CTGF mRNA levels in MCF7-R. a-c Graphs represent the mean ± SD of at least three independent triplicate experiments. * denotes statistically significant values compared with (b) positive control or (c) MCF7 cells (*adjp<0.05; **adjp<0.001). # denotes statistically significant values compared with untreated MCF7-R (#pval<0.05)