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Fig. 8 | BMC Cancer

Fig. 8

From: Serotoninergic receptor ligands improve Tamoxifen effectiveness on breast cancer cells

Fig. 8

Effect of SER79 and SER68 on Tamoxifen-cultured MCF7 cells. MCF7 cells were cultured in presence of Tamoxifen (1 μM) for 10 days. Estrogen-starved MCF7 and Tamoxifen-cultured MCF7 cells (48 h) were (a-c) treated with SER79 (20 μM, 5 μM) or SER68 (40 μ, 20 μM), (b) treated with Tamoxifen (5 μM) in presence of SER79 (20 μM, 5 μM) or SER68 (40 μM, 20 μM). All treatments were carried out in presence of E2 (100 nM) for 72 h. As positive control, the cells were treated with (a-c) E2 alone or (b) E2 in absence or in presence of SER (as indicated, dotted line). a,b Cell viability was assessed by sulforhodamine B assay (see Methods). The results were reported as percentage of viable cells compared to positive control, considered as maximum viability (100%). c mRNA levels of CTGF were determined by qPCR (see Methods and Table 1). Data were normalized on Ribosomal Protein S23 (Rps23) gene as internal standard and reported as CTGF mRNA levels in Tamoxifen-cultured MCF7 relative to those in MCF7 cells (dotted line). a-c Graphs represent the mean ± SD of at least three independent triplicate experiments. * denotes statistically significant values compared with (a,b) positive control (*adjp<0.05, ** adjp<0.01) or (c) MCF7 cells (*pval<0.05); # denotes statistically significant values compared with (b) cells treated with Tamoxifen in absence of SER or (c) untreated Tamoxifen-cultured MCF7 cells (#pval<0.01, ##pval<0.0001)

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