Fig. 6
From: Serotoninergic receptor ligands improve Tamoxifen effectiveness on breast cancer cells
Effect of SER on MCF7 cell cycle and Tamoxifen response. a MCF7 cells were treated with SER137, SER142, SER167, SER195, SER196, SER198, SER79, SER177, SER31, SER68 at IC50 doses. After 72 h, cell cycle was assessed by Propidium Iodide staining (see Methods). The results were reported as percentage of cells in G0/G1, S and G2/M cell cycle phase. b Estrogen-starved MCF7 cells (48 h) were treated with Tamoxifen (5 μM) in presence of E2 (100nM) and selected SER (SER137, SER142, SER167, SER195, SER196, SER79, SER177, SER31, SER68) at IC50 dose. As positive control, the cells were treated with E2 and SER alone (without Tamoxifen, dotted line). After 72 h, cell viability was assessed by sulforhodamine B assay (see Methods). The results were reported as percentage of viable cells compared to positive control, considered as maximum viability (100%). c Estrogen-starved MCF7 cells (48 h) were treated with selected SER at IC50 doses in presence of E2 (100nM). After 72 h, mRNA levels of CTGF were determined by qPCR (see Methods and Table 1). Data were normalized on Ribosomal Protein S23 (Rps23) gene as internal standard and were reported as CTGF mRNA levels in MCF7 treated with SER relative to those in untreated cells (dotted line). a-c Data represent the mean ± SD of at least three independent triplicate experiments. * denotes statistically significant values compared with (a) percentage of untreated cells in G0/G1, S and G2/M cell cycle phase (*pval<0.05,**pval<0.01), (b) positive control (*adjp<0.05,**adjp <0.01,***adjp<0.001), (c) untreated cells (pval<0.05); # denotes statistically significant values compared with cells treated with Tamoxifen in absence of SER (#pval<0.05)