Fig. 3

Chromatin Accessibility in FOX motifs and Signature 2 Regions Associate with Tumor Progression Stages. A-B) Heatmaps (A) and profile plots (B) showing accessibility in signatures 1, 2 and 3 across tumor stages. Heatmaps have regions ordered from greatest to least average accessibility across tumor stages, regions with no signal are ignored in calculation of average accessibility. C) Boxplots of accessibility in signatures 1, 2 and 3 across tumor stages, indicating that only signature 2 shows an accessibility trend across stages. D) Boxplot comparing accessibility of FOX motifs in accessible peak regions (n=96280) by tumor stage. E) Screenshots from IGV depicting average accessibility of tumor stages and FOXA1 binding in MCF-7 cells from ChIP-Seq in regions within each chromatin signature. ChIP-Seq data is Log2FC over control. F) Boxplot comparing FOXA1 expression levels across tumor stages. P-values in C, D and F obtained from one-tailed parametric t-tests. * is p<0.01, ** is p<0.001, *** is p<0.0001.