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Fig. 7 | BMC Cancer

Fig. 7

From: MiR-509-3p is oncogenic, targets the tumor suppressor PHLPP2, and functions as a novel tumor adjacent normal tissue based prognostic biomarker in colorectal cancer

Fig. 7

Overexpression of miR-509-3p can be laterally transferred from stably transduced CRC cell lines to a normal colon cell line. A Schematic of the experimental transfer of culture media from HCT116 or SW480 CRC cell lines stably transduced with miR-509-3p (pre-mir) or empty vector control through a 0.45 μm filter which serves as a conditioned media for the growth of CCD841CoN, a normal colon cell line. B CCD841CoN showed a sharp increase in the expression of miR-509-3p upon being cultured in the conditioned media from HCT116 or SW480 stably transduced with miR-509-3p, as compared to the empty vector control group. C Lateral transfer of miR-509-3p from stably transduced HCT116 or SW480 to CCD841CoN resulted in an increase in the proliferation rates of the normal cell line as compared to the empty vector group. Assessment of the proliferation rates were performed using a BrdU assay. *p value < 0.05.**p value < 0.01

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