Fig. 6

Experimental validation by gastric cancer cells. A MGC803 cell was knockdown of ADAMDEC1 gene and western blot was applied to detect the expression level of ADAMDEC1. B MTT assay was used to detect the cell proliferation rates in 0 h, 24 h, 48 h and 72 h. Data are means ± SD in three independent experiment (*P < 0.05). C Transwell assay was performed to detect the migration of MGC803 cell after silencing ADAMDEC1 for 48 h. Data are means ± SD in three independent experiment (*P < 0.05). D mRNA expression level change of PD-L1 in silencing ADAMDEC1 of MGC803 cell. E Western blot was used to detect the change of PD-L1 expression level in MGC803 cell with ADAMDEC1 knockdown. F Jurkat T cells were co-incubated with ADAMDEC1-NC and ADAMDEC1-KD MGC803 cell for 48 h, respectively. The apoptosis in Jurkat T cells was measured by flow cytometry analysis