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Fig. 4 | BMC Cancer

Fig. 4

From: Hsa_circ_0008434 regulates USP9X expression by sponging miR-6838-5p to promote gastric cancer growth, migration and invasion

Fig. 4

Hsa_circ_0008434 regulates USP9X expression via miR-6838. A The potential binding sites between USP9X and miR-6838-5p were predicted with the miRanda database and MiRDB website. B Luciferase reporter assays showed that USP9X cotransfection with miR-6838-5p mimics led to decreased luciferase activity. C The relative expression of USP9X was examined by immunohistochemical staining analysis. D A wound healing assay was conducted to explore the migration ability of SGC7901 cells with or without USP9X knockdown. E Transwell assays were performed to examine the invasion ability of SGC7901 cells with or without USP9X knockdown. F A colony formation assay was performed to identify the colony formation ability of SGC7901 cells with or without USP9X knockdown. G qRT-PCR was performed to identify miR-6838’s negative regulation of USP9X by hsa_circ_0008434. H Western blot analysis was performed to prove that hsa_circ_0008434 regulates USP9X expression via miR-6838. *p < 0.05; **p < 0.01; NS, no significance

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