Fig. 3

Hsa_circ_0008434 serves as a miRNA sponge for miR-6838-5p. A A miRNA-circRNA-gene regulatory network was established via the miRanda database. B RNA-FISH was carried out to assess the colocalization of hsa_circ_0008434 and miR-6838-5p. Blue represents DAPI nuclear staining, red represents hsa_circ_0008434 staining and green represents miR-6838 staining. C Pearson correlation analysis was used to determine the correlation of hsa_circ_0008434 expression with miR-6838-5p in GC tissues. D The potential binding sites between hsa_circ_0008434 and miR-6838-5p were determined via the MiRanda database. E A dual-luciferase reporter assay was performed in HEK-293 T cells, and hsa_circ_0008434 cotransfection with miR-6838-5p mimics led to decreased luciferase activity. F A wound healing assay was conducted to explore the migration ability of SGC7901 cells with or without miR-6838 upregulation. G Transwell assays were performed to examine the invasion ability of SGC7901 cells with or without miR-6838 upregulation. H A colony formation assay was performed to assess the colony formation ability of SGC7901 cells with or without miR-6838 upregulation. ^*p < 0.05; ^**p < 0.01; NS, no significance