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Fig. 3 | BMC Cancer

Fig. 3

From: TRAP1 suppresses oral squamous cell carcinoma progression by reducing oxidative phosphorylation metabolism of Cancer-associated fibroblasts

Fig. 3

Label-free proteomics reveals that proteins mainly involved in metabolic pathways are differentially expressed in the mitochondria of CAFs and NFs. A Contamination and abundance of mitochondria preparations obtained by kit protocol was assessed by Western blotting. ML, mitochondrial lysate; WCL, whole cell lysate; B Integrity of purified mitochondria preparations were assessed by transmission electron microscopy. The image showed well- preserved mitochondria with electrondense matrix and cristae (red arrowheads). C Gene ontology (GO) annotation of differential proteins (CAFs/NFs) in mitochondria. D Kyoto Encyclopedia of Genes and Genomes (KEGG) annotation of differential proteins (CAFs/NFs) in mitochondria. E Volcano plot of a total of 183 differential protein in mitochondria (CAFs/NFs). The − log10 (P value) was plotted against the log2 (FC), FC: CAFs/NFs. The red dots represented proteins up-regulated in mitochondria (CAFs/NFs), green dots corresponded to proteins down-regulated in mitochondria (CAFs/NFs). F Heatmap of a total of 76 differential protein (CAFs/NFs) related to mitochondria. G Immunofluorescence and Immunohistochemistry analysis of ATP5O and TRAP1 expression in CAFs of OSCC and para-OSCC tissue. Data are presented as mean ± SD of three independent experiments, and n.s represents no significant difference. Statistically significant relationships are indicated by *p < 0.05

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