Fig. 2

Immune cell composition, signaling pathway enrichment and expression of immune checkpoints among NMF subgroups. A Heat map of immune cell proportion revealed distinct composition among four NMF subgroups; B A higher proportion of CD8 T cells, activated CD4 memory T cells, and M1 macrophages was found in the NMF2 subgroup. Comparisons were made between NMF2A and NMF1, and between NMF2B and NMF3, separately; C The single sample gene set variation analysis (ssGSEA) showing enriched signaling pathways in four NMF subgroups; D Overall survival (OS) analysis indicated no significant difference in these immune groups based on the NMF classification; E The relative expression of six immune checkpoints was higher in the NMF2 subgroup. Comparisons were made between NMF2A and NMF1, and between NMF2B and NMF3, separately; F Significant associations between the expression of immune checkpoints and several immune cell types were identified in the TCGA database and several GEO datasets, where red dots indicated positive association and blue negative. The higher the coefficient, the stronger the association. ****P < 0.0001